U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX20525320: GSM7430904: Barcode dataset of sample 4; Mustela putorius furo; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 76.3M spots, 10.2G bases, 3Gb downloads

External Id: GSM7430904_r1
Submitted by: Mayerlab, Max Planck Institute of Neurobiology.
Study: Barcode lineage tracking of ferret cortical progeny using TrackerSeq (Mayer lab)
PRJNA976704 • SRP439808 • All experiments • All runs
show Abstracthide Abstract
Diversity of cortical radial glia cells (RGCs) and their complex relationships to generate neurons in species with expanded germinal zones and a folded cortex, remains unclear. We used TrackerSeq, a technique that integrates DNA barcodes into the genome of electroporated RGCs, to identify the distinct cell lineages that shape ferret cortex. Overall design: E34 ferret embryos were electroporated with a piggyBac transposon-based library and eGFP+ cells were isolated at E37 by FACS and procesed for their parallel transcriptomic and barcode-lineage tracing characterization.
Sample: Barcode dataset of sample 4
SAMN35439747 • SRS17834764 • All experiments • All runs
Organism: Mustela putorius furo
Library:
Name: GSM7430904
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Embryonic tissue was collected in ice-cold Leibowitz medium and papain dissociation was carried out (Wortington, #LK003150). GFP-possitive cells were FACS sorted and after processed on the 10x Genomic Platform. Gel Beads-in-emulsion and cDNA generation carrying cell- and transcript-specific barcode was done using the Chromium Single Cell 3' Reagent Kit v3.1 (PN-1000121) following the manufacturer's protocol. Transcriptomic libraries were prepared for sequencing using standard Illumina protocols. Barcode libraries were prepared from PCR cDNA to amplify barcode regions with custumed primers with standard Illumina adapters.
Runs: 2 runs, 76.3M spots, 10.2G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR2474864637,929,7275.1G1.5Gb2024-03-27
SRR2474864738,363,5355.1G1.5Gb2024-03-27

ID:
27949029

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...